A reliable and reproducible experimental challenge model for peste des petits ruminants virus.
نویسندگان
چکیده
Experimental challenge protocols that consistently reproduce clinical signs of peste des petits ruminants in Alpine goats infected with a tissue culture-passaged peste des petits ruminants virus are described. The protocols can be used to carry out quality-controlled vaccine efficacy and pathogenesis studies under experimental conditions.
منابع مشابه
Cloning of fusion (F) protein gene of peste des petits ruminants virus (PPRV) in secretory Pichia pastoris vector
With advent and development of DNA recombinant technology and advantages of p. pastoris expression system, fusion (F) protein of PPRV expression, because of effective immunodominant role could be an appropriate candidate for production of recombinant vaccine against PPR disease. In this study, F gene of PPRV Nigeria 75/1 strain (1637 bp) was amplified using RT-PCR and purified. It was then cl...
متن کاملSeroprevalence Study of Peste Des Petits Ruminants in Sheeps of Shabestar, Iran
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Figure. Distribution of ruminants in Eritrea with peste des petits ruminants virus infection, 2003–2011. Colored circles indicate regions from which tissue samples were collected from goats and sheep during outbreaks of peste des petits ruminants; nucleotide sequences for the samples were determined and analyzed.
متن کاملDetermination of the minimum fully protective dose of adenovirus-based DIVA vaccine against peste des petits ruminants virus challenge in East African goats.
Peste des petits ruminants virus (PPRV) causes an economically important disease of sheep and goats, primarily in developing countries. It is becoming the object of intensive international control efforts. Current vaccines do not allow vaccinated and infected animals to be distinguished (no DIVA capability). We have previously shown that recombinant, replication-defective, adenovirus expressing...
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 50 11 شماره
صفحات -
تاریخ انتشار 2012